Detection of Legionella species in environmental water by the quantitative PCR method in combination with ethidium monoazide treatment.

نویسندگان

  • Hiroaki Inoue
  • Tomoko Takama
  • Miwa Yoshizaki
  • Kunio Agata
چکیده

We detected Legionella species in 111 bath water samples and 95 cooling tower water samples by using a combination of conventional plate culture, quantitative polymerase chain reaction (qPCR) and qPCR combined with ethidium monoazide treatment (EMA-qPCR) methods. In the case of bath water samples, Legionella spp. were detected in 30 samples by plate culture, in 85 samples by qPCR, and in 49 samples by EMA-qPCR. Of 81 samples determined to be Legionella-negative by plate culture, 56 and 23 samples were positive by qPCR and EMA-qPCR, respectively. Therefore, EMA treatment decreased the number of Legionella-positive bath water samples detected by qPCR. In contrast, EMA treatment had no effect on cooling tower water samples. We therefore expect that EMA-qPCR is a useful method for the rapid detection of viable Legionella spp. from bath water samples.

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عنوان ژورنال:
  • Biocontrol science

دوره 20 1  شماره 

صفحات  -

تاریخ انتشار 2015